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The production of recombinant human EEF1A1 protein involves manipulating the expression of the desired gene in E.coli cells. The sequence (1-462aa) of foreign DNA is merged with an expression vector along with the N-terminal 6xHis-tag gene and subsequently introduced into E.coli cells. Positive cells are chosen and cultivated to induce the expression of the desired protein. The resulting recombinant human EEF1A1 protein undergoes purification through affinity purification, with a purity exceeding 85%, as confirmed by SDS-PAGE.
EEF1A1, a member of the eukaryotic elongation factor family, is responsible for delivering aminoacyl-tRNA to the ribosome during translation [1]. It is important in protein synthesis. This protein plays a significant role in peptide chain extension, promoting protein synthesis and regulating the elongation phase of translation [2]. EEF1A1 can also regulate the cell cycle, proliferation, and apoptosis [3].
EEF1A1 is associated with various diseases and conditions. For instance, studies have linked EEF1A1 to cancer progression, with overexpression of EEF1A1 being correlated with poor prognosis in colorectal cancer and hepatocellular carcinoma [4][5]. EEF1A1 inhibits apoptosis and chemotherapy sensitivity in certain cancer types [6].
References:
[1] Q. Hu, L. Ning, Z. Yu, G. Dou, & L. Li, Proteomic identification of eef1a1 as a molecular target of curcumol for suppressing metastasis of mda-mb-231 cells, Journal of Agricultural and Food Chemistry, vol. 65, no. 14, p. 3074-3082, 2017. https://doi.org/10.1021/acs.jafc.7b00573
[2] X. Liu, L. Chen, G. Jin, C. Yan, Z. Huang, J. Huet al., The ubiquitin-like protein fat10 stabilizes eef1a1 expression to promote tumor proliferation in a complex manner, Cancer Research, vol. 76, no. 16, p. 4897-4907, 2016. https://doi.org/10.1158/0008-5472.can-15-3118
[3] S. Xu, W. XiaoQian, X. Zhang, C. Chen, H. Chen, & F. She, Caga orchestrates eef1a1 and pkcδ to induce interleukin-6 expression in helicobacter pylori-infected gastric epithelial cells, Gut Pathogens, vol. 12, no. 1, 2020. https://doi.org/10.1186/s13099-020-00368-3
[4] A. Fan, X. Zhao, H. Líu, D. Li, T. Guo, J. Zhanget al., eef1a1 promotes colorectal cancer progression and predicts poor prognosis of patients, Cancer Medicine, vol. 12, no. 1, p. 513-524, 2022. https://doi.org/10.1002/cam4.4848
[5] S. Chen, S. Lu, L. Liu, C. Wang, X. Yang, Z. Zhanget al., Eef1a1 overexpression enhances tumor progression and indicates poor prognosis in hepatocellular carcinoma, Translational Oncology, vol. 11, no. 1, p. 125-131, 2018. https://doi.org/10.1016/j.tranon.2017.11.001
[6] Á. Blanch, F. Robinson, I. Watson, L. Cheng, & M. Irwin, Eukaryotic translation elongation factor 1-alpha 1 inhibits p53 and p73 dependent apoptosis and chemotherapy sensitivity, Plos One, vol. 8, no. 6, p. e66436, 2013. https://doi.org/10.1371/journal.pone.0066436
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